Fluo-4 AM

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Intracellular calcium response of Jurkat cells to stimulation with A23187. Jurkat cells were stained with 5 μM BD Pharmingen™ Fluo-4 AM (Cat. No. 565878) in DPBS with calcium and magnesium for 20 minutes at 37°C. Cells were pelleted once and further incubated in complete medium for 30 minutes to allow complete hydrolysis of AM moieties. Cells were then pelleted and resuspended in DPBS with calcium and magnesium and analyzed on an BD Accuri™ C6 in the FL-1 channel. After 30 seconds of acquisition, 1 μM calcium ionophore A23187 (left, Sigma Aldrich Cat. No. C7522) or DMSO vehicle (right) was added to the tube, followed by mixing with the pipette. Jurkat cells show an increase in intracellular calcium concentration with addition of calcium ionophore A23187, but not with addition of DMSO vehicle.

BD PBX, a highly sensitive calcium probe allows ratiometric analysis and antibody labeling of primary cells. (A) Comparison of excitation and emission spectra of Fluo-4-AM and the calcium indicator included in the

Fluo-4 AM *Ultrapure Grade*, CAS 273221-67-3, AAT Bioquest

Fluo-4 - Wikipedia

PhenoVue Fluo-4 AM, Calcium Indicator

Quest Fluo-8(TM), AM, AAT Bioquest

A comparative assessment of fluo Ca2+ indicators in rat ventricular myocytes. - Abstract - Europe PMC

Mag-Fluo-4 within the ER can reliably measure Ca 2+ uptake and release.

Frontiers High-throughput screening method for discovering CatSper inhibitors using membrane depolarization caused by external calcium chelation and fluorescent cell barcoding

Fluorescent Ca2+ indicators directly inhibit the Na,K-ATPase and disrupt cellular functions

Fluo-4, AM, cell permeant

Fluo-4 AM (Fluo-4 Acetoxymethyl ester, CAS Number: 273221-67-3)

Reliable measurement of free Ca2+ concentrations in the ER lumen using Mag- Fluo-4.,Cell Calcium - X-MOL